TNF alpha antibody

Catalog No. ABIN2689930

Cited in 4+ publications. The Mouse Monoclonal anti-TNF alpha antibody (Clone MAb11) (ABIN2689930) specifically detects TNF alpha in ICS. The antibody is reactive with Rhesus Monkey, Baboon and Cynomolgus samples.

Quick Overview for TNF alpha antibody (ABIN2689930)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Rhesus Monkey, Baboon, Cynomolgus

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Intracellular Staining (ICS)

Clone: MAb11

Product Details anti-TNF alpha Antibody

Characteristics: BD Pharmingen™ Purified Mouse Anti-Human TNF - Purified - Clone MAb11 - Isotype Mouse IgG1, κ - Reactivity Bab, Cyno, Rhe - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Human TNF

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-TNF alpha antibody (ABIN2689930)

Sopper, Stahl-Hennig, Demuth, Johnston, Dörries, ter Meulen: "Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys." in: Cytometry, Vol. 29, Issue 4, pp. 351-62, (1998) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Verdier, Aujoulat, Condevaux, Descotes: "Determination of lymphocyte subsets and cytokine levels in cynomolgus monkeys." in: Toxicology, Vol. 105, Issue 1, pp. 81-90, (1996) (PubMed).

Rathjen, Cowan, Furphy, Aston: "Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors." in: Molecular immunology, Vol. 28, Issue 1-2, pp. 79-86, (1991) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome