TNF alpha antibody

Catalog No. ABIN2689936

Cited in 6+ publications. The Armenian Hamster Monoclonal anti-TNF alpha antibody (Clone TN3) (ABIN2689936) specifically detects TNF alpha in WB, ELISA, IP, Neut and Inhibition. The antibody is reactive with Mouse, Rat and Rabbit samples.

Quick Overview for TNF alpha antibody (ABIN2689936)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse, Rat, Rabbit

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Neutralization (Neut), Blocking Antibody (Inhibition)

Clone: TN3

Product Details anti-TNF alpha Antibody

Characteristics: The TN3-19.12 antibody reacts with rat and mouse tumor-necrosis factor (TNF) proteins (also known as TNF-α) . Moreover, the TN3-19.12 antibody is reported to crossreact with rabbit TNF, but it does not crossreact with mouse lymphotoxin-α (LT-α, also known as TNF-β) nor with human TNF. The immunogen used to generate the TN3-19.12 hybridoma was E. coli-expressed, purified recombinant mouse TNF protein. This monoclonal antibody has been reported to neutralize the bioactivities of mouse, rat and rabbit TNF. This antibody is routinely tested by ELISA. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Hamster Anti-Mouse/Rat TNF - Purified - Clone TN3-19.12 - Isotype Armenian Hamster IgG1, λ1 - Reactivity Ms, Rat, Rab - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant mouse TNF protein

Isotype: IgG1 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-TNF alpha antibody (ABIN2689936)

Takahashi, Kapás, Fang, Krueger: "An anti-tumor necrosis factor antibody suppresses sleep in rats and rabbits." in: Brain research, Vol. 690, Issue 2, pp. 241-4, (1996) (PubMed).

Ferran, Dautry, Mérite, Sheehan, Schreiber, Grau, Bach, Chatenoud: "Anti-tumor necrosis factor modulates anti-CD3-triggered T cell cytokine gene expression in vivo." in: The Journal of clinical investigation, Vol. 93, Issue 5, pp. 2189-96, (1994) (PubMed).

Rabinovici, Bugelski, Esser, Hillegass, Griswold, Vernick, Feuerstein: "Tumor necrosis factor-alpha mediates endotoxin-induced lung injury in platelet activating factor-primed rats." in: The Journal of pharmacology and experimental therapeutics, Vol. 267, Issue 3, pp. 1550-7, (1994) (PubMed).

Leiby, Fortier, Crawford, Schreiber, Nacy: "In vivo modulation of the murine immune response to Francisella tularensis LVS by administration of anticytokine antibodies." in: Infection and immunity, Vol. 60, Issue 1, pp. 84-9, (1992) (PubMed).

Merrick, He, Craig, Clark, Corsini, Rosenthal, Mansfield, Selkirk: "Two dimensional gel electrophoresis of cellular and secreted proteins from rat alveolar macrophages after lipopolysaccharide treatment." in: Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society, Vol. 2, Issue 6, pp. 177-87, (1992) (PubMed).

Sheehan, Ruddle, Schreiber: "Generation and characterization of hamster monoclonal antibodies that neutralize murine tumor necrosis factors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 11, pp. 3884-93, (1989) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome