CSTB antibody

Catalog No. ABIN271833

Product Details anti-CSTB Antibody

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSTB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Stefin B protein. (region surrounding asn84)

Cross-Reactivity (Details): Species reactivity (tested):Human and Rat.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate Buffered Saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CSTB antibody (ABIN271833)

Penna, Cerciello, Chambery, Russo, Cernilogar, Pedone, Perrone-Capano, Cappello, Di Giaimo, Crispino: "Cystatin B Involvement in Synapse Physiology of Rodent Brains and Human Cerebral Organoids." in: Frontiers in molecular neuroscience, Vol. 12, pp. 195, (2019) (PubMed).

Target Details for CSTB

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Alternative Name: Cystatin-B (CSTB Products)

Synonyms: CST6 antibody, EPM1 antibody, EPM1A antibody, PME antibody, STFB antibody, ULD antibody, CSTB antibody, Cyb antibody, Epm1 antibody, Stfb antibody, MGC189005 antibody, pme antibody, cst6 antibody, epm1 antibody, stfb antibody, AA960480 antibody, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 antibody, ATCYS6 antibody, ATCYSB antibody, cystatin B antibody, LOC100008600 antibody, LOC100136235 antibody, CPI1 antibody, cystatin B antibody, cystatin B (stefin B) antibody, cystatin 14a, tandem duplicate 2 antibody, cystatin-B antibody, CSTB antibody, Cstb antibody, cstb antibody, cst14a.2 antibody, CYSB antibody, cpi1 antibody, LOC101123265 antibody

Background: Cystatin A (also designated STF1, STFA, stefin A or cystatin AS) and cystatin B (also designated PME, CST6, STFB, CPI-B, stefin B and liver thiol proteinase inhibitor) are thiol protease inhibitors that form complexes with Papain and the cathepsins B, H and L. Cystatin A, a cytoplasmic protein, is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Cystatin B protects against intracellular proteases leaking out of lysosomes and is primarily expressed in heart, liver and kidney.Synonyms: CPI-B, CST6, CSTB, Liver thiol proteinase inhibitor, STFB, Stefin-B

Molecular Weight: approx. 11 kDa

Gene ID: 1476

NCBI Accession: NP_000091

UniProt: P04080

Pathways: Response to Water Deprivation