CD40 antibody
-
- Target See all CD40 Antibodies
- CD40
-
Reactivity
- Human, Mouse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This CD40 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of CD40 protein. (region surrounding Gln259)
- Cross-Reactivity (Details)
- Species reactivity (tested):Human, Mouse.
- Purification
- Affinity Chromatography using epitope-specific immunogen.
- Top Product
- Discover our top product CD40 Primary Antibody
-
-
- Application Notes
-
ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- Target
- CD40
- Alternative Name
- CD40 (CD40 Products)
- Synonyms
- Bp50 antibody, CDW40 antibody, TNFRSF5 antibody, p50 antibody, AI326936 antibody, GP39 antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TRAP antibody, Tnfrsf5 antibody, TNFSF5 antibody, CD40 molecule antibody, CD40 antigen antibody, CD40 antibody, Cd40 antibody
- Background
- Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. A receptor ligand pair central to the transmission of this signal is CD40, expressed on the surface of B cells, together with CD40L, expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. B cells are sensitive to these cytokines only subsequent to CD40/CD40L-driven DNA synthesis. A downstream mediator of the CD40 signaling pathway, designated CRAF, is a member of an expanding family of proteins that contain a conserved cysteine- and histidine-rich RING finger motif. Other members of the family include TRAF1 and TRAF2. The latter proteins have been shown to regulate TNF-R2 as well as CD40 signaling through activation of the NFkB family of transcription factors.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5
- Molecular Weight
- approx. 30 kDa
- Gene ID
- 958
- NCBI Accession
- NP_001241
- UniProt
- P25942
- Pathways
- NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
-