XRCC4 antibody

Catalog No. ABIN271982

The Rabbit Polyclonal anti-XRCC4 antibody is suitable to detect XRCC4 in samples from Human. It has been validated for WB.

Quick Overview for XRCC4 antibody (ABIN271982)

Target: XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XRCC4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-XRCC4 Antibody

Specificity: This antibody detects endogenous levels of XRCC4 protein. (region surrounding asn291)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. Western blot: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for XRCC4

Target: XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))

Alternative Name: XRCC4

Background: The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a 70 kDa protein that facilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNA polymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair. XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.Synonyms: DNA repair protein XRCC4, X-ray repair cross-complementing protein 4

Molecular Weight: approx. 38 kDa

Gene ID: 7518

NCBI Accession: NP_003392

UniProt: Q13426

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response