F2R
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
6A7H10
unconjugated
Application Notes
ELISA: 1/10000approx. 1/20000. Western blot: 1/500approx. 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Thrombin receptor (also designated protease-activated receptor-1 or PAR-1), PAR-2 and PAR-3 compose a distinct class of G protein-coupled receptors activated by proteolysis. Cleavage of these receptors by proteases occurs within the amino-terminal extracellular domain. Thrombin, a serine protease involved in platelet aggregation and blood coagulation, activates the thrombin receptor, resulting in elevated intracellular calcium levels in platelets. Thrombin also cleaves PAR-3 in vitro, suggesting that PAR-3 may be involved in thrombosis or mitogenesis. Thrombin receptor and PAR-4 appear to account for most thrombin signaling in platelets. Activation of PAR-2 in vitro is induced by trypsin, suggesting that PAR-2 is not an alternative thrombin receptor. Cytokines including TNF-alpha and IL-1beta increase PAR-2 expression, indicating PAR-2 involvement in the acute inflammatory response.Synonyms: CF2R, Coagulation factor II receptor, PAR-1, PAR1, Proteinase-activated receptor 1