Sp2 antibody

Catalog No. ABIN272208

Product Details anti-Sp2 Antibody

Target: Sp2 (Sp2 Transcription Factor (Sp2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sp2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Sp2 protein. (region surrounding Asp512)

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Sp2

Target: Sp2 (Sp2 Transcription Factor (Sp2))

Alternative Name: SP2 (Sp2 Products)

Synonyms: 4930480I16Rik antibody, mKIAA0048 antibody, sp2 antibody, Sp2 transcription factor antibody, Sp2 transcription factor S homeolog antibody, SP2 antibody, Sp2 antibody, sp2.S antibody

Background: The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4(SPR-1). Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3, but not Sp2 or Sp4, interact with E2, a regulatory element for the b4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription. The gene3 encoding human Sp2 maps to chromosome 17p32.3.Synonyms: KIAA0048, Transcription factor Sp2

Molecular Weight: approx. 64 kDa

Gene ID: 6668

NCBI Accession: NP_003101

UniProt: Q02086