PD-L1 antibody (PE)
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- Target See all PD-L1 Antibodies
- PD-L1 (CD274 (PD-L1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PD-L1 antibody is conjugated to PE
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Application
- Flow Cytometry (FACS), ELISA
- Specificity
- Recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.
- Purification
- Protein A purified
- Immunogen
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Recombinant human CD274(B7-H1).
Type of Immunogen: Recombinant protein - Clone
- ANC6H1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PD-L1 Primary Antibody
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- Application Notes
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Approved: ELISA, Flo
Usage: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 μg/mL PHA. 5 x 105 per tube were washed and pre incubated 5 minutes with 20^l of 250^g/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-human CD274 at a dilution factor of 1:50 (20 ^g/mL). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD274 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 50 mM sodium phosphate, pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 4°C. Do not freeze. Protect from light.
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- Target
- PD-L1 (CD274 (PD-L1))
- Alternative Name
- B7-H1 / PD-L1 / CD274 (PD-L1 Products)
- Synonyms
- B7-H antibody, B7H1 antibody, PD-L1 antibody, PDCD1L1 antibody, PDCD1LG1 antibody, PDL1 antibody, A530045L16Rik antibody, B7h1 antibody, Pdcd1l1 antibody, Pdcd1lg1 antibody, Pdl1 antibody, RGD1566211 antibody, CD274 molecule antibody, CD274 antigen antibody, programmed cell death 1 ligand 2 antibody, CD274 antibody, Cd274 antibody, PDCD1LG2 antibody
- Background
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Name/Gene ID: CD274
Synonyms: CD274, B7-H, B7 homolog 1, B7-H1, B7H1, CD274 Molecule, CD274 antigen, PDCD1 ligand 1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed death ligand 1 - Gene ID
- 29126
- Pathways
- Cancer Immune Checkpoints
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