RUNX2 antibody (AA 244-258)

Catalog No. ABIN2738787

The Rabbit Polyclonal anti-RUNX2 antibody has been validated for WB. It is suitable to detect RUNX2 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit, Monkey, Hamster, Chimpanzee, Gibbon, Orang-Utan and Sheep.

Quick Overview for RUNX2 antibody (AA 244-258) (ABIN2738787)

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit, Monkey, Hamster, Chimpanzee, Gibbon, Orang-Utan, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RUNX2 Antibody

Binding Specificity: AA 244-258

Specificity: Human CBFA1 / RUNX2

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human RUNX2(244-258aa DRLSDLGRIPHPSMR). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Ferret, Sheep, Hamster, Elephant, Rabbit, Horse, Pig, Guinea pig, Dog (100%), Turkey, Sparrow, Chicken, Platypus, Lizard (93%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.5 - 1 μg/mL)

Usage: Core binding factor A1(CBFA1/RUNX2) is a runt-like transcription factor essential for osteoblast differentiation. This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. D'Souza et al.(1999) indicate a non-redundant role for Cbfa1 in tooth development that may be distinct from that in bone formation. In odontogenesis, Cbfa1 is not involved in the early signaling networks regulating tooth initiation and early morphogenesis but regulates key epithelial-mesenchymal interactions that control advancing morphogenesis and histodifferentiation of the epithelial enamel organ.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS, 2.5 % BSA, 0.025 % sodium azide/thimerosal.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 1 month or -20°C for longer. Avoid freeze/thaw cycles.

Target Details for RUNX2

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Alternative Name: CBFA1 / RUNX2

Background: Name/Gene ID: RUNX2

Synonyms: RUNX2, AML3, CCD, CCD1, OSF2, PEBP2A1, PEBP2A2, PEA2-alpha A, PEBP2-alpha A, OSF-2, PEBP2A, PEBP2aA1, CBF-alpha-1, CBFA1, Oncogene AML-3, PEA2aA, PEBP2aA

Gene ID: 860