CD11c antibody (APC)
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- Target See all CD11c (ITGAX) Antibodies
- CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD11c antibody is conjugated to APC
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Application
- Flow Cytometry (FACS)
- Specificity
- Integrin alpha X subunit (CD11c) of about 150 kd.
- Purification
- Protein A purified
- Immunogen
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Human rheumatoid synovial fluid cells/monocytes.
Type of Immunogen: Cells - Clone
- 3-9
- Isotype
- IgG1
- Top Product
- Discover our top product ITGAX Primary Antibody
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- Application Notes
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Approved: Flo
Usage: Five x 105 ficoll prepared human PBMC were washed and pre incubated 10 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-CD11c/APC at a 1:50 dilution (10 μg/mL). Cells were washed three times, fixed and analyzed by FACS. An 11 % sub population of the cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a Mouse IgG1/APC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti- CD11c antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 50 mM sodium phosphate, pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 4°C. Do not freeze. Protect from light.
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- Target
- CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))
- Alternative Name
- ITGAX / CD11c (ITGAX Products)
- Synonyms
- CD11C antibody, SLEB6 antibody, RGD1561123 antibody, AI449405 antibody, Cd11c antibody, Cr4 antibody, N418 antibody, integrin subunit alpha X antibody, integrin alpha X antibody, ITGAX antibody, Itgax antibody
- Background
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Name/Gene ID: ITGAX
Family: Integrin
Synonyms: ITGAX, CD11c antigen, Integrin alpha-X, Leu M5, alpha subunit, p150 95 integrin alpha chain, SLEB6, CD11C, Leu M5 - Gene ID
- 3687
- Pathways
- Complement System, Activated T Cell Proliferation, Integrin Complex
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