PD-1 antibody

Catalog No. ABIN2743525

Product Details anti-PD-1 Antibody

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Anti-CD279 antibody clone ANC4H6 binds to CD279 present on PHA activated PBMC in FACS, and binds to recombinant CD279 in EIA. Pre incubation of recombinant CD279-coated plates with ANC4H6 blocks binding of biotinylated recombinant CD273 in EIA.

Purification: Protein A purified

Immunogen: Recombinant human CD279.

Type of Immunogen: Recombinant protein

Clone: ANC4H6

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo

Usage: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated 5 days in culture with 5 μg/mL Phytohemagglutanin (PHA). 5 x 10⁵ per tube were pre incubated 10 minutes with 250 μg/mL human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-CD279 antibody at 10 μg/mL. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.6 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Binding was blocked when antibody was pre incubated with a 10-fold excess of recombinant CD279-muIg.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/mL Gentamicin sulfate.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PDCD1 / CD279 / PD-1 (PDCD1 Products)

Synonyms: CD279 antibody, PD-1 antibody, PD1 antibody, SLEB2 antibody, hPD-1 antibody, hPD-l antibody, Ly101 antibody, Pdc1 antibody, programmed cell death 1 antibody, PDCD1 antibody, Pdcd1 antibody

Background: Name/Gene ID: PDCD1
Family: Apoptosis

Synonyms: PDCD1, CD279, HPD-1, HPD-l, PD1, Protein PD-1, SLEB2, PD-1, CD279 antigen, Programmed cell death 1

Gene ID: 5133

Pathways: Cancer Immune Checkpoints