WNT3A antibody (Biotin)

Catalog No. ABIN465635

Product Details anti-WNT3A Antibody (Biotin)

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT3A antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Specificity: Human WNT-3a

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human WNT-3a.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hWNT-3a by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hWNT-3a. To detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human WNT-3a (LS-B8210) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hWNT-3a. Western Blot: To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for WNT3A

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Alternative Name: WNT3A (WNT3A Products)

Synonyms: WNT3A antibody, Wnt-3a antibody, vt antibody, Zwnt[a] antibody, wnt3 antibody, wnt3 l antibody, wnt3l antibody, wnt[a] antibody, Xwnt-3a antibody, wnt-3a antibody, wnt3a-A antibody, xwnt3a antibody, WNT-3A antibody, WNT3 antibody, wingless-type MMTV integration site family, member 3A antibody, Wnt family member 3A antibody, wingless-type MMTV integration site family member 3A L homeolog antibody, WNT3A antibody, Wnt3a antibody, wnt3a antibody, wnt3a.L antibody

Background: Name/Gene ID: WNT3A
Family: WNT

Synonyms: WNT3A, Protein Wnt-3a

Gene ID: 89780

UniProt: P56704

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity