TEK antibody (Extracellular Domain)

Catalog No. ABIN2745570

The Mouse Monoclonal anti-TEK antibody is suitable to detect TEK in samples from Human. It has been validated for WB, ELISA and FACS.

Quick Overview for TEK antibody (Extracellular Domain) (ABIN2745570)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TEK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone: Tek2

Product Details anti-TEK Antibody

Binding Specificity: Extracellular Domain

Specificity: Native human TIE-2 in ELISA experiments and on the surface of different human cell types.

Cross-Reactivity: Human

Purification: Protein G purified.

Immunogen: Recombinant human soluble extracellular domain of TIE-2.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Expiry Date: 6 months

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: TIE-2

Background: TIE-2 acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. It has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. It is required for normal angiogenesis and heart development during embryogenesis and for post-natal hematopoiesis. After birth, it activates or inhibits angiogenesis, depending on the context. It inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1 and ultimately to the stimulation of sprouting angiogenesis.

UniProt: Q02763

Pathways: RTK Signaling, Growth Factor Binding