ROR2 antibody (AA 900-950)

Catalog No. ABIN2747364

Product Details anti-ROR2 Antibody

Target: ROR2 (Receptor Tyrosine Kinase-Like Orphan Receptor 2 (ROR2))

Binding Specificity: AA 900-950

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Specificity: The antibody labels a strong band of ROR2 at 113 kDa in ABIN1741697 samples and in other cancer cell lines.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Does not cross react to any other cellular protein.

Characteristics: ROR2- selective antibodies were generated against a peptide taken the C-terminal region. The ROR2-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The anti- ROR1-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides ROR2 Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for ROR2 antibody is also available, please inquire before ordering.

Purification: Affinity Purified

Immunogen: Synthetic peptide taken within amino acid region 900-950 on human ROR2 protein.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Synonyms: neurotrophic tyrosine kinase receptor-related 2/ NTRKR2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55-0.75 μg/μL

Handling Advice: Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.

Storage: -20 °C

Storage Comment: For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.

Target Details for ROR2

Target: ROR2 (Receptor Tyrosine Kinase-Like Orphan Receptor 2 (ROR2))

Alternative Name: ROR2 (ROR2 Products)

Synonyms: BDB antibody, BDB1 antibody, NTRKR2 antibody, Ntrkr2 antibody, mRor2 antibody, ROR2 antibody, bdb antibody, bdb1 antibody, Xror2 antibody, ntrkr2 antibody, MGC97773 antibody, LOC100219935 antibody, ror2 antibody, xror2 antibody, receptor tyrosine kinase like orphan receptor 2 antibody, receptor tyrosine kinase-like orphan receptor 2 antibody, receptor tyrosine kinase like orphan receptor 2 L homeolog antibody, ROR2 antibody, Ror2 antibody, ror2 antibody, ror2.L antibody

Background: The Receptor tyrosine kinase-like Orphan Receptor (ROR) proteins are conserved tyrosine kinase receptors that pattern tissues and organs during embryonic development and in adulthood. ROR family proteins are ligand-dependent transcription factors that are required for skeletal and neuronal development, and modulate cell migration, cell polarity, and convergent extension. RORs act as Wnt receptors signaling via novel non-canonical Wnt pathways mediated by the adapter protein disheveled and the non-receptor tyrosine kinase Src. RORs can either activate or repress Wnt target expression depending on the cellular context and can also modulate signal transduction by sequestering Wnt ligands away from their signaling receptors.The mammalian ROR family of receptor tyrosine kinases consists of two structurally related proteins, ROR1 and ROR2. ROR2 (receptor tyrosine kinase-like orphan receptor 2), also known as neurotrophic tyrosine kinase receptor-related 2 (NTRKR2), is a single pass trans membrane tyrosine-protein kinase receptor. It contains a cytoplasmic tyrosine kinase domain, distally located serine-threonine-rich domains, an extracellular immunoglobulin-like domain, a cysteine-rich domain and a kringle domain. ROR2 is important for skeletal and endocrine development and is required for cartilage and growth plate development. It promotes the differentiation of osteoblasts and plays an important role in the early formation of chondrocytes. ROR2 also interacts with canonical Wnt1 and Wnt3, regulating their signaling pathways. Defects in ROR2 can result in the autosomal dominant skeletal disorder, brachydactylic type B1, a skeletal disorder characterized by hypoplasia/aplasia of distal phalanges and nails or the autosomal recessive skeletal disorder, Robinow syndrome which is characterized by skeletal dysplasia with generalized limb bone shortening, segmental defects of the spine, brachydactyly, and a dysmorphic facial appearance. ROR2 possesses dual roles in tumorigenesis as a tumor suppressor or activator depending on tumor type. ROR2 expression in primary human Renal Cell Carcinoma (RCC) tumors showed a significant correlation with several Wnt signaling genes, including the classical feedback target gene Axin2.

NCBI Accession: NP_004551

UniProt: Q01974

Pathways: RTK Signaling, WNT Signaling