RFC5 antibody (N-Term)

Catalog No. ABIN2773876

The Rabbit Polyclonal anti-RFC5 antibody (ABIN2773876) specifically detects RFC5 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Saccharomyces cerevisiae and Zebrafish (Danio rerio) samples.

Quick Overview for RFC5 antibody (N-Term) (ABIN2773876)

Target: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RFC5 Antibody

Binding Specificity: N-Term

Purpose: RFC5 Antibody - N-terminal region

Sequence: METSALKQQE QPAATKIRNL PWVEKYRPQT LNDLISHQDI LSTIQKFINE

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rat: 100%, Yeast: 90%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against RFC5. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RFC5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8110293

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RFC5

Target: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Alternative Name: RFC5

Background:

Targeted Subunit: 5

Background Information: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Gene Name: Replication factor C (activator 1) 5, 36.5 kDa

Alternative Symbols: RFC36

Protein Name: Replication factor C subunit 5

Molecular Weight: 38kDa

Gene ID: 5985

NCBI Accession: NP_031396

UniProt: P40937

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA