RUNX2 antibody (Middle Region)

Catalog No. ABIN2774905

This Rabbit Polyclonal antibody specifically detects RUNX2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig and Rabbitand has been mentioned in 1 publication.

Quick Overview for RUNX2 antibody (Middle Region) (ABIN2774905)

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RUNX2 Antibody

Binding Specificity: Middle Region

Sequence: DTATSDFCLW PSTLSKKSQA GASELGPFSD PRQFPSISSL TESRFSNPRM

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RUNX2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RUNX2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 521 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RUNX2 antibody (ABIN2774905)

Schuchardt, Tölle, Prüfer, Prüfer, Huang, Jankowski, Jankowski, Zidek, van der Giet: "Uridine adenosine tetraphosphate activation of the purinergic receptor P2Y enhances in vitro vascular calcification." in: Kidney international, Vol. 81, Issue 3, pp. 256-65, (2012) (PubMed).

Target Details for RUNX2

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Alternative Name: RUNX2

Background: RUNX2 is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis, acting as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants, encoding different protein isoforms, result from alternate promoter use as well as alternate splicing.
Alias Symbols: CCD, AML3, CCD1, OSF2, CBFA1, OSF-2, PEA2aA, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1
Protein Interaction Partner: HDAC7, HDAC6, TP53, UBC, RB1, PML, HIF1A, SMURF1, SMAD1, SMURF2, HIVEP3, WWP1, FHL2, UBTF, SUV39H1, HDAC1, STAT3, SMAD3, RBM14, SOX9, NR0B2, EP300, HDAC5, HDAC4, HDAC3, CREBBP, XRCC5, HES1, XRCC6, BMPR1A, KAT2B, TLE1, YAP1, KAT6B, TAF1A, AXIN1, SMAD6, MAP
Protein Size: 521

Molecular Weight: 57 kDa

Gene ID: 860

NCBI Accession: NM_001024630, NP_001019801

UniProt: Q13950