BARD1 antibody (C-Term)

Catalog No. ABIN2775649

This Rabbit Polyclonal antibody specifically detects BARD1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Cow, Rabbit and Zebrafish (Danio rerio).

Quick Overview for BARD1 antibody (C-Term) (ABIN2775649)

Target: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Cow, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BARD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BARD1 Antibody

Binding Specificity: C-Term

Purpose: BARD1 Antibody - C-terminal region

Sequence: LFDGCYFYLW GTFKHHPKDN LIKLVTAGGG QILSRKPKPD SDVTQTINTV

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against BARD1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human BARD1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42953-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for BARD1

Target: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Alternative Name: BARD1

Background:

Background Information: This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Name: BRCA1 associated RING domain 1

Protein Name: BRCA1-associated RING domain protein 1

Molecular Weight: 74kDa

Gene ID: 580

NCBI Accession: NP_000456

UniProt: F6MDH8

Pathways: DNA Damage Repair