Insulin Receptor antibody (Middle Region)

Catalog No. ABIN2776774

This Rabbit Polyclonal antibody specifically detects Insulin Receptor in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit and Pig.

Quick Overview for Insulin Receptor antibody (Middle Region) (ABIN2776774)

Target: Insulin Receptor (INSR)

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Insulin Receptor Antibody

Binding Specificity: Middle Region

Sequence: ENNVVHLMWQ EPKEPNGLIV LYEVSYRRYG DEELHLCVSR KHFALERGCR

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against INSR. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human INSR

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1382 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: INSR

Background: This receptor binds insulin and has a tyrosine-protein kinase activity. Isoform Short has a higher affinity for insulin. INSR mediates the metabolic functions of insulin. INSR binding to insulin stimulates association of the receptor with downstream media
Alias Symbols: CD220, HHF5
Protein Interaction Partner: KRT31, UBC, ARRB2, MOK, Grb2, Hgf, DOK1, SH2B1, Stat5b, TEAD1, STAT5A, JAK2, JAK1, IRS1, IRF7, PLCG1, GRB10, Socs6, Socs1, SOCS3, SH2B2, HGS, INPPL1, Calmodulin, Mad2l1, KRT27, DOK5, DOK4, SNX6, SNX4, SNX2, ACP1, PTPN2, SYNCRIP, FRS2, PIK3R3, VAV3, ARHGAP
Protein Size: 1382

Molecular Weight: 154 kDa

Gene ID: 3643

NCBI Accession: NM_000208, NP_000199

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity