Aurora A antibody (C-Term)

Catalog No. ABIN2777171

The Rabbit Polyclonal anti-Aurora A antibody is suitable to detect Aurora A in samples from Human, Cow and Sheep. It has been validated for WB and IHC.

Quick Overview for Aurora A antibody (C-Term) (ABIN2777171)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human, Cow, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Aurora A Antibody

Binding Specificity: C-Term

Purpose: AURKA Antibody - C-terminal region

Sequence: ARDLISRLLK HNPSQRPMLR EVLEHPWITA NSSKPSNCQN KESASKQS

Predicted Reactivity: Cow: 80%, Human: 100%, Sheep: 80%

Characteristics: This is a rabbit polyclonal antibody against AURKA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human AURKA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP30876-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: AURKA

Background:

Background Information: AURKA is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. It is found at the centrosome in interphase cells and at the spindle poles in mitosis. This protein may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.

Gene Name: Aurora kinase A

Alternative Symbols: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, PPP1R47

Protein Name: Aurora kinase A

Molecular Weight: 46kDa

Gene ID: 6790

NCBI Accession: NP_003591

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization