FLJ11730 (N-Term) antibody

Catalog No. ABIN2777884

Product Details

Target: FLJ11730

Binding Specificity: N-Term

Reactivity: Human, Cow, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Sequence: HNKAAPPQIP DTRRELAELV KRKQELAETL ANLERQIYAF EGSYLEDTQM

Predicted Reactivity: Cow: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against FLJ11730. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FLJ11730

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 201 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: FLJ11730

Background: The screening of cDNA expression libraries from human tumors with serum antibody (SEREX) has proven to be a powerful method for identifying the repertoire of tumor antigens recognized by the immune system of cancer patients, referred to as the cancer immunome. In this regard, cancer/testis (CT) antigens are of particular interest because of their immunogenicity and restricted expression patterns. Synoivial sarcomas are striking with regard to CT antigen expression, however, highly expressed in sarcoma, CT antigens do not induce frequent humoral immune responses in sarcoma patients. Sera from two patients were used to immunoscreen cDNA libraries from two synovial sarcoma cell lines and normal testis, resulting in the identification of 113 distinct antigens. Sarcoma antigen NY-SAR-91 is one of them.
Alias Symbols: Eaf6, CENP-28, C1orf149, CDABP0189, NY-SAR-91, RP3-423B22.2, FLJ11730
Protein Interaction Partner: PLEKHF2, TRIM54, LDOC1, UBC, FXR2, H2AFZ, ELAVL1, SUMO2, JADE1, KAT5, tat, MRGBP, ING5, KAT6A, BRPF1, CCDC85B, L3MBTL2, ING3, ING4, KAT7,
Protein Size: 201

Molecular Weight: 23 kDa

Gene ID: 64769

NCBI Accession: NM_022756, NP_073593

UniProt: Q86WE3