ID4 antibody (Middle Region)

Catalog No. ABIN2779567

The Rabbit Polyclonal anti-ID4 antibody (ABIN2779567) specifically detects ID4 in WB. The antibody is reactive with Human, Mouse, Rat, Pig, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse and Rabbit samples.

Quick Overview for ID4 antibody (Middle Region) (ABIN2779567)

Target: ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4))

Reactivity: Human, Mouse, Rat, Pig, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ID4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ID4 Antibody

Binding Specificity: Middle Region

Purpose: ID4 Antibody - middle region

Sequence: CYSRLRRLVP TIPPNKKVSK VEILQHVIDY ILDLQLALET HPALLRQPPP

Predicted Reactivity: Cow: 85%, Dog: 100%, Guinea Pig: 85%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 79%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against ID4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ID4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32327-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ID4

Target: ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4))

Alternative Name: ID4

Background:

Background Information: Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins.Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Inhibitor of DNA binding 4, dominant negative helix-loop-helix protein

Alternative Symbols: IDB4, bHLHb27

Protein Name: DNA-binding protein inhibitor ID-4

Molecular Weight: 16kDa

Gene ID: 3400

NCBI Accession: NP_001537

UniProt: P47928