RUNX2 antibody (C-Term)

Catalog No. ABIN2780589

There is 1 publication for this product available. The Rabbit Polyclonal anti-RUNX2 antibody is suitable to detect RUNX2 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Cow, Horse, Zebrafish (Danio rerio), Pig and Rabbit. It has been validated for WB.

Quick Overview for RUNX2 antibody (C-Term) (ABIN2780589)

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Cow, Horse, Zebrafish (Danio rerio), Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RUNX2 Antibody

Binding Specificity: C-Term

Purpose: RUNX2 Antibody - C-terminal region

Sequence: TTTSNGSTLL NPNLPNQNDG VDADGSHSSS PTVLNSSGRM DESVWRPY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 83%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against RUNX2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RUNX2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38453-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RUNX2 antibody (ABIN2780589)

Wang, He, Liu, Liu, Zhou, Hao, Sun, Wang: "Synergistic effects of overexpression of BMP‑2 and TGF‑β3 on osteogenic differentiation of bone marrow mesenchymal stem cells." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5514-5520, (2016) (PubMed).

Target Details for RUNX2

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Alternative Name: RUNX2

Background:

Background Information: RUNX2 is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis, acting as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants, encoding different protein isoforms, result from alternate promoter use as well as alternate splicing.This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing.

Gene Name: Runt-related transcription factor 2

Alternative Symbols: CCD, AML3, CCD1, CLCD, OSF2, CBFA1, OSF-2, PEA2aA, PEBP2aA, CBF-alpha-1

Protein Name: Runt-related transcription factor 2

Molecular Weight: 55kDa

Gene ID: 860

NCBI Accession: NP_004339

UniProt: Q13950