PIGA antibody (Middle Region)

Catalog No. ABIN2782189

There is 1 publication for this product available. The Rabbit Polyclonal anti-PIGA antibody is suitable to detect PIGA in samples from Human, Mouse, Rat, Cow, Pig, Rabbit and Dog. It has been validated for WB.

Quick Overview for PIGA antibody (Middle Region) (ABIN2782189)

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIGA Antibody

Binding Specificity: Middle Region

Purpose: PIGA Antibody - middle region

Sequence: SVKSLCEGLE KAIFQLKSGT LPAPENIHNI VKTFYTWRNV AERTEKVYDR

Predicted Reactivity: Cow: 86%, Dog: 93%, Human: 100%, Mouse: 93%, Pig: 79%, Rabbit: 79%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against PIGA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PIGA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP45344-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PIGA antibody (ABIN2782189)

Traulsen, Pacheco, Dingli: "On the origin of multiple mutant clones in paroxysmal nocturnal hemoglobinuria." in: Stem cells (Dayton, Ohio), Vol. 25, Issue 12, pp. 3081-4, (2007) (PubMed).

Target Details for PIGA

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Alternative Name: PIGA

Background:

Targeted Subunit: A

Background Information: PIGA is a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene.This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized.

Gene Name: Phosphatidylinositol glycan anchor biosynthesis, class A

Alternative Symbols: GPI3, PNH1, PIG-A, MCAHS2

Protein Name: Phosphatidylinositol N-acetylglucosaminyltransferase subunit A Ensembl ENSP00000416102

Molecular Weight: 19kDa

Gene ID: 5277

NCBI Accession: NP_065206

UniProt: B3KUV7

Pathways: Inositol Metabolic Process