GOT1 antibody (N-Term)

Catalog No. ABIN2783280

This Rabbit Polyclonal antibody specifically detects GOT1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Goat, Guinea Pig, Horse, Rabbit and Saccharomyces cerevisiae. It has been mentioned in 3+ publications

Quick Overview for GOT1 antibody (N-Term) (ABIN2783280)

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Goat, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GOT1 Antibody

Binding Specificity: N-Term

Purpose: GOT1 Antibody - N-terminal region

Sequence: MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV

Predicted Reactivity: Cow: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 92%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against GOT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GOT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48205-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-GOT1 antibody (ABIN2783280)

Povlsen, Løfgren, Dalgas, Birkler, Johannsen, Støttrup, Bøtker: "Protection against myocardial ischemia-reperfusion injury at onset of type 2 diabetes in Zucker diabetic fatty rats is associated with altered glucose oxidation." in: PLoS ONE, Vol. 8, Issue 5, pp. e64093, (2013) (PubMed).

Takeda, Ishida, Takahashi, Ueda: "Synaptic vesicles are capable of synthesizing the VGLUT substrate glutamate from ?-ketoglutarate for vesicular loading." in: Journal of neurochemistry, Vol. 121, Issue 2, pp. 184-96, (2012) (PubMed).

Inoue, Matsumoto, Miyoshi, Kobayashi: "Elevated liver enzymes in women with a family history of diabetes." in: Diabetes research and clinical practice, Vol. 79, Issue 3, pp. e4-7, (2008) (PubMed).

Target Details for GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: GOT1

Background:

Background Information: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enz

Gene Name: Glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)

Alternative Symbols: AST1, cCAT, GIG18, cAspAT, ASTQTL1

Protein Name: Aspartate aminotransferase, cytoplasmic

Molecular Weight: 46kDa

Gene ID: 2805

NCBI Accession: NP_002070

UniProt: P17174

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process