GOT1 antibody (N-Term)

Catalog No. ABIN2783281

There is 1 publication for this product available. The Rabbit Polyclonal anti-GOT1 antibody is suitable to detect GOT1 in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Goat, Guinea Pig, Horse, Rabbit and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for GOT1 antibody (N-Term) (ABIN2783281)

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Goat, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GOT1 Antibody

Binding Specificity: N-Term

Sequence: MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV

Predicted Reactivity: Cow: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 92%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against GOT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GOT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 413 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GOT1 antibody (ABIN2783281)

Inoue, Matsumoto, Miyoshi, Kobayashi: "Elevated liver enzymes in women with a family history of diabetes." in: Diabetes research and clinical practice, Vol. 79, Issue 3, pp. e4-7, (2008) (PubMed).

Target Details for GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: GOT1

Background: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: GIG18, ASTQTL1
Protein Interaction Partner: UBC, CHRAC1, SBDS, UBE2H, TYMS, MTAP, IDH1, HPRT1, ANXA6, ABI3BP, EGFR, TMEM120A, PC,
Protein Size: 413

Molecular Weight: 46 kDa

Gene ID: 2805

NCBI Accession: NM_002079, NP_002070

UniProt: P17174

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process