Manic Fringe antibody (Middle Region)

Catalog No. ABIN2783425

The Rabbit Polyclonal anti-Manic Fringe antibody is suitable to detect Manic Fringe in samples from Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig and Rabbit. It has been validated for WB.

Quick Overview for Manic Fringe antibody (Middle Region) (ABIN2783425)

Target: Manic Fringe (MFNG) (MFNG)

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Manic Fringe antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Manic Fringe Antibody

Binding Specificity: Middle Region

Purpose: MFNG Antibody - middle region

Sequence: MAPWASGSRF MDTSALIRLP DDCTMGYIIE CKLGGRLQPS PLFHSHLETL

Predicted Reactivity: Cow: 79%, Dog: 86%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MFNG. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MFNG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48572-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Manic Fringe

Target: Manic Fringe (MFNG) (MFNG)

Alternative Name: MFNG

Background:

Background Information: MFNG is one of the evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene is a member of the fringe gene family which also includes Radical and Lunatic fringe. They all encode evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta1,3 N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Alternative Symbols: -

Protein Name: Beta-1,3-N-acetylglucosaminyltransferase manic fringe

Molecular Weight: 36kDa

Gene ID: 4242

NCBI Accession: NP_002396

UniProt: O00587

Pathways: Notch Signaling