FHIT antibody (Middle Region)

Catalog No. ABIN2784442

Product Details anti-FHIT Antibody

Target: FHIT (Fragile Histidine Triad (FHIT))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Zebrafish (Danio rerio), Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHIT antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VHVLPRKAGD FHRNDSIYEE LQKHDKEDFP ASWRSEEEMA AEAAALRVYF

Predicted Reactivity: Dog: 93%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 79%, Rabbit: 93%, Rat: 79%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against FHIT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FHIT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 147 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for FHIT

Target: FHIT (Fragile Histidine Triad (FHIT))

Alternative Name: FHIT (FHIT Products)

Synonyms: AP3Aase antibody, FRA3B antibody, FHIT antibody, zgc:73176 antibody, AW045638 antibody, Fra14A2 antibody, LOC100223655 antibody, fragile histidine triad antibody, fragile histidine triad gene antibody, fragile histidine triad protein antibody, fragile histidine triad L homeolog antibody, FHIT antibody, Fhit antibody, fhit antibody, PY07476 antibody, fhit.L antibody

Background: This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene.
Alias Symbols: AP3Aase, FRA3B
Protein Interaction Partner: FHIT, REL, MDM2, UBC, RAB40B, TRIM23, LEF1, CTNNB1, UBE2I, SRC, GSN, HSPD1, FDXR,
Protein Size: 147

Molecular Weight: 17 kDa

Gene ID: 2272

NCBI Accession: NM_002012, NP_002003

UniProt: P49789