E-cadherin antibody (Middle Region)

Catalog No. ABIN2784498

The Rabbit Polyclonal anti-E-cadherin antibody is suitable to detect E-cadherin in samples from Human, Mouse, Rat, Pig, Dog, Rabbit, Cow, Horse and Zebrafish (Danio rerio). It has been validated for WB, IHC and IF.

Quick Overview for E-cadherin antibody (Middle Region) (ABIN2784498)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human, Mouse, Rat, Pig, Dog, Rabbit, Cow, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-E-cadherin Antibody

Binding Specificity: Middle Region

Sequence: QEITSYTAQE PDTFMEQKIT YRIWRDTANW LEINPDTGAI STRAELDRED

Predicted Reactivity: Cow: 93%, Dog: 79%, Horse: 93%, Human: 100%, Mouse: 79%, Pig: 93%, Rabbit: 93%, Rat: 93%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against CDH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CDH1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 882 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1

Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: Arc-1, CD324, CDHE, ECAD, LCAM, UVO
Protein Interaction Partner: UBC, MDM2, UCHL3, CDC27, PTEN, CTNNB1, PIP5K1C, CTNND1, CBLL1, RNF2, GMNN, LMNA, KRT18, KRT9, KRT8, KRT1, IGHA1, HSPD1, HSP90AA1, HSPA9, HSPA8, HSPA5, GBAS, CTNNA1, CRYAB, CFTR, C1QBP, ACTN4, ACADVL, ANP32B, NIPSNAP1, XRCC5, TGM2, SKP2, SET, RAD17, POLR2E
Protein Size: 882

Molecular Weight: 80 kDa

Gene ID: 999

NCBI Accession: NM_004360, NP_004351

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation