Crossover junction endonuclease EME1 (EME1) (N-Term) antibody

Catalog No. ABIN2785183

The Rabbit Polyclonal anti-Crossover junction endonuclease EME1 antibody (ABIN2785183) specifically detects Crossover junction endonuclease EME1 in WB. The antibody is reactive with Human, Rat, Mouse, Pig, Cow, Dog, Guinea Pig, Horse and Rabbit samples.

Quick Overview for Crossover junction endonuclease EME1 (EME1) (N-Term) antibody (ABIN2785183)

Target: Crossover junction endonuclease EME1 (EME1)

Reactivity: Human, Rat, Mouse, Pig, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Purpose: EME1 Antibody - N-terminal region

Sequence: MALKKSSPSL DSGDSDSEEL PTFAFLKKEP SSTKRRQPER EEKIVVVDIS

Predicted Reactivity: Cow: 85%, Dog: 93%, Guinea Pig: 92%, Horse: 86%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against EME1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EME1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52987-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: Crossover junction endonuclease EME1 (EME1)

Alternative Name: EME1

Background:

Background Information: EME1 and MUS81 (MIM 606591) form an endonuclease complex that cleaves branched DNA structures, especially those arising during stalled DNA replication.EME1 and MUS81 (MIM 606591) form an endonuclease complex that cleaves branched DNA structures, especially those arising during stalled DNA replication (Abraham et al., 2003 [PubMed 14609959]).[supplied by OMIM].

Gene Name: Essential meiotic endonuclease 1 homolog 1 (S. pombe)

Alternative Symbols: MMS4L, SLX2A

Protein Name: Crossover junction endonuclease EME1

Molecular Weight: 63kDa

Gene ID: 146956

NCBI Accession: NP_689676

UniProt: Q96AY2