Aurora A antibody (Middle Region)

Catalog No. ABIN2785452

This Rabbit Polyclonal antibody specifically detects Aurora A in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Sheep, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for Aurora A antibody (Middle Region) (ABIN2785452)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Aurora A Antibody

Binding Specificity: Middle Region

Purpose: AURKA Antibody - middle region

Sequence: REKQSKFILA LKVLFKAQLE KAGVEHQLRR EVEIQSHLRH PNILRLYGYF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%, Sheep: 77%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against AURKA. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human AURKA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP53574-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: AURKA

Background:

Background Information: AURKA is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. It is found at the centrosome in interphase cells and at the spindle poles in mitosis. This protein may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.

Gene Name: aurora kinase C

Alternative Symbols: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, PPP1R47

Protein Name: Aurora kinase A

Molecular Weight: 44kDa

Gene ID: 6790

NCBI Accession: NP_940839

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization