ARAF antibody (Middle Region)

Catalog No. ABIN2785788

The Rabbit Polyclonal anti-ARAF antibody (ABIN2785788) specifically detects ARAF in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Pig, Sheep, Dog, Rabbit and Horse samples.

Quick Overview for ARAF antibody (Middle Region) (ABIN2785788)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivity: Human, Mouse, Rat, Cow, Pig, Sheep, Dog, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ARAF Antibody

Binding Specificity: Middle Region

Sequence: PRGSPSPASV SSGRKSPHSK SPAEQRERKS LADDKKKVKN LGYRDSGYYW

Predicted Reactivity: Cow: 100%, Dog: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against ARAF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ARAF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 606 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: ARAF

Background: ARAF belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily. It contains 1 phorbol-ester/DAG-type zinc finger, 1 protein kinase domain, and 1 RBD (Ras-binding) domain. ARAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Alias Symbols: A-RAF, ARAF1, PKS2, RAFA1
Protein Interaction Partner: AGTRAP, KRAS, CDC37, MAP2K1, GNA12, FKBP5, UBC, RNF2, FBXO6, YWHAB, HIPK4, YWHAE, RRAS2, BAD, TEKT4P2, WNK1, NELFCD, DIDO1, TESK1, MAP2K2, MLH1, HSP90AA1, E2F6, BRAF, TIRAP, IRF7, IRAK2, YWHAG, MYO18A, CHD6, YWHAZ, PBK, NUDT14, KLHL12, COPS3, TIMM44, ASS1
Protein Size: 606

Molecular Weight: 67 kDa

Gene ID: 369

NCBI Accession: NM_001654, NP_001645

UniProt: P10398

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C