ARAF antibody (Middle Region)

Catalog No. ABIN2785788

This anti-ARAF antibody is a Rabbit Polyclonal antibody detecting ARAF in WB and IHC. Suitable for Human, Mouse, Rat, Cow, Pig, Sheep, Dog, Rabbit and Horse.

Quick Overview for ARAF antibody (Middle Region) (ABIN2785788)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivity: Human, Mouse, Rat, Cow, Pig, Sheep, Dog, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ARAF Antibody

Binding Specificity: Middle Region

Sequence: PRGSPSPASV SSGRKSPHSK SPAEQRERKS LADDKKKVKN LGYRDSGYYW

Predicted Reactivity: Cow: 100%, Dog: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against ARAF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ARAF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 606 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: ARAF

Background: ARAF belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily. It contains 1 phorbol-ester/DAG-type zinc finger, 1 protein kinase domain, and 1 RBD (Ras-binding) domain. ARAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Alias Symbols: A-RAF, ARAF1, PKS2, RAFA1
Protein Interaction Partner: AGTRAP, KRAS, CDC37, MAP2K1, GNA12, FKBP5, UBC, RNF2, FBXO6, YWHAB, HIPK4, YWHAE, RRAS2, BAD, TEKT4P2, WNK1, NELFCD, DIDO1, TESK1, MAP2K2, MLH1, HSP90AA1, E2F6, BRAF, TIRAP, IRF7, IRAK2, YWHAG, MYO18A, CHD6, YWHAZ, PBK, NUDT14, KLHL12, COPS3, TIMM44, ASS1
Protein Size: 606

Molecular Weight: 67 kDa

Gene ID: 369

NCBI Accession: NM_001654, NP_001645

UniProt: P10398

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C