POLB antibody (Middle Region)

Catalog No. ABIN2786683

The Rabbit Polyclonal anti-POLB antibody (ABIN2786683) specifically detects POLB in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Dog, Goat, Guinea Pig, Horse and Rabbit samples.

Quick Overview for POLB antibody (Middle Region) (ABIN2786683)

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Dog, Goat, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-POLB Antibody

Binding Specificity: Middle Region

Purpose: POLB Antibody - middle region

Sequence: GPSAARKFVD EGIKTLEDLR KNEDKLNHHQ RIGLKYFGDF EKRIPREEML

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against POLB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLB

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56406-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: POLB

Background:

Background Information: In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. Also see POLA (MIM 312040).

Gene Name: Polymerase (DNA directed), beta

Alternative Symbols: MGC125976

Protein Name: DNA polymerase beta

Molecular Weight: 38kDa

Gene ID: 5423

NCBI Accession: NP_002681

UniProt: P06746

Pathways: DNA Damage Repair