RASGRF1 antibody (N-Term)

Catalog No. ABIN2786747

The Rabbit Polyclonal anti-RASGRF1 antibody has been validated for WB. It is suitable to detect RASGRF1 in samples from Mouse, Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for RASGRF1 antibody (N-Term) (ABIN2786747)

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Reactivity: Mouse, Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASGRF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RASGRF1 Antibody

Binding Specificity: N-Term

Sequence: RELDNNRSAL SAASAFAIAT AGANEGTPNK EKYRRMSLAS AGFPPDQRNG

Specificity: Predicted to recognized both human RASGRF1 isoforms (145, 55kD)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 85%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against RASGRF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RASGRF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 489 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RASGRF1

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Alternative Name: RASGRF1

Background: The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein.
Alias Symbols: CDC25, CDC25L, GNRP, GRF1, GRF55, H-GRF55, PP13187, ras-GRF1
Protein Interaction Partner: EHD4, SNRNP70, CEBPA, ADRBK1, MYC, UBC, TNK2, YWHAE, USP8, RAC1, PRKACA, NTRK2, RRAS2, NTRK3, NTRK1, RHOA, YWHAB, PPP1R9B, MARK3, HRAS,
Protein Size: 489

Molecular Weight: 54 kDa

Gene ID: 5923

NCBI Accession: NM_153815, NP_722522

Pathways: RTK Signaling, Cell Division Cycle, M Phase