Retinoblastoma 1 antibody (N-Term)

Catalog No. ABIN2787534

This anti-Retinoblastoma 1 antibody is a Rabbit Polyclonal antibody detecting Retinoblastoma 1 in WB. Suitable for Human, Mouse, Rat, Cow, Rabbit and Pig.

Quick Overview for Retinoblastoma 1 antibody (N-Term) (ABIN2787534)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Retinoblastoma 1 Antibody

Binding Specificity: N-Term

Sequence: MPPKTPRKTA ATAAAAAAEP PAPPPPPPPE EDPEQDSGPE DLPLVRLEFE

Predicted Reactivity: Cow: 93%, Human: 100%, Mouse: 80%, Pig: 93%, Rabbit: 93%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against RB1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 928 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: RB1

Background: The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Alias Symbols: OSRC, RB, pRb, pp110, p105-Rb
Protein Interaction Partner: RAF1, E2F1, ABL1, HDAC1, KDM2A, SMARCA4, USP7, UBC, DYRK1A, NEFM, DYRK1B, MAGEA11, ENC1, PPP1CA, CDKN1C, CDKN1A, CDK6, CDK4, CDK2, CCNE1, CCNA2, CCND1, HDAC11, PAX5, HIF1A, E2F4, CEBPE, CCNA1, SUMO1, UBE2I, TFAP2A, CDK3, CDK1, CCNC, CCNB1, MRPS18B, SUMO2,
Protein Size: 928

Molecular Weight: 106 kDa

Gene ID: 5925

NCBI Accession: NM_000321, NP_000312

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy