Calpain S1 antibody (Middle Region)

Catalog No. ABIN2787678

Cited in 1 publication. The Rabbit Polyclonal anti-Calpain S1 antibody (ABIN2787678) specifically detects Calpain S1 in WB. The antibody is reactive with Human, Mouse, Rat, Rabbit, Cow, Pig, Dog, Guinea Pig, Horse and Sheep samples.

Quick Overview for Calpain S1 antibody (Middle Region) (ABIN2787678)

Target: Calpain S1 (CAPNS1) (Calpain, Small Subunit 1 (CAPNS1))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Pig, Dog, Guinea Pig, Horse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpain S1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Calpain S1 Antibody

Binding Specificity: Middle Region

Purpose: CAPNS1 Antibody - middle region

Sequence: RRYSDESGNM DFDNFISCLV RLDAMFRAFK SLDKDGTGQI QVNIQEWLQL

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against CAPNS1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CAPNS1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58434-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Calpain S1 antibody (ABIN2787678)

Kulkarni, Liu, Qi, Zhu, Klinke, Liu: "Differential proteomic analysis of caveolin-1 KO cells reveals Sh2b3 and Clec12b as novel interaction partners of caveolin-1 and Capns1 as a potential mediator of caveolin-1-induced apoptosis." in: The Analyst, Vol. 138, Issue 22, pp. 6986-96, (2013) (PubMed).

Target Details for Calpain S1

Target: Calpain S1 (CAPNS1) (Calpain, Small Subunit 1 (CAPNS1))

Alternative Name: CAPNS1

Background:

Targeted Subunit: 1

Background Information: Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. This gene encodes a small subunit common to both calpain I and II and is associated with myotonic dystrophy. Two transcript variants encoding the same protein have been identified for this gene.

Gene Name: Calpain, small subunit 1

Alternative Symbols: CANP, CDPS, CSS1, CANPS, CAPN4, CALPAIN4

Protein Name: Calpain small subunit 1

Molecular Weight: 29kDa

Gene ID: 826

NCBI Accession: NP_001003962

UniProt: P04632

Pathways: Apoptosis