Calpain S1 antibody (Middle Region)

Catalog No. ABIN2787678

Product Details anti-Calpain S1 Antibody

Target: Calpain S1 (CAPNS1) (Calpain, Small Subunit 1 (CAPNS1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Pig, Dog, Guinea Pig, Horse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpain S1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RRYSDESGNM DFDNFISCLV RLDAMFRAFK SLDKDGTGQI QVNIQEWLQL

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against CAPNS1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CAPNS1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 268 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Calpain S1 antibody (ABIN2787678)

Kulkarni, Liu, Qi, Zhu, Klinke, Liu: "Differential proteomic analysis of caveolin-1 KO cells reveals Sh2b3 and Clec12b as novel interaction partners of caveolin-1 and Capns1 as a potential mediator of caveolin-1-induced apoptosis." in: The Analyst, Vol. 138, Issue 22, pp. 6986-96, (2013) (PubMed).

Target Details for Calpain S1

Target: Calpain S1 (CAPNS1) (Calpain, Small Subunit 1 (CAPNS1))

Alternative Name: CAPNS1 (CAPNS1 Products)

Synonyms: 30K antibody, CALPAIN4 antibody, CANP antibody, CANPS antibody, CAPN4 antibody, CDPS antibody, CSS1 antibody, Capn4 antibody, CAPNS1 antibody, Capa-4 antibody, Capa4 antibody, D7Ertd146e antibody, MGC84330 antibody, capns1 antibody, cb616 antibody, wu:fb81g08 antibody, wu:fl09e04 antibody, zgc:110603 antibody, calpain small subunit 1 antibody, calpain, small subunit 1 antibody, calpain, small subunit 1 L homeolog antibody, calpain, small subunit 1 a antibody, CAPNS1 antibody, Capns1 antibody, capns1.L antibody, capns1a antibody

Background: Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. This gene encodes a small subunit common to both calpain I and II and is associated with myotonic dystrophy. Two transcript variants encoding the same protein have been identified for this gene.
Alias Symbols: 30K, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1
Protein Interaction Partner: AGTRAP, HUWE1, NEDD1, TUBGCP2, TUBGCP3, CDC20, UBC, OGFOD1, STK26, ISOC1, DCPS, MAT2B, PROSC, FERMT2, YWHAQ, PDIA4, YWHAZ, YWHAH, YWHAG, YWHAE, PTMA, PPP1CA, PAK2, PAFAH1B1, MYO1E, MAT2A, GNAI3, GAPDH, EIF5, TBCB, ALDH7A1, ASNS, APEX1, ADRB2, PPARGC1A, VC
Protein Size: 268

Molecular Weight: 29 kDa

Gene ID: 826

NCBI Accession: NM_001003962, NP_001003962

UniProt: P04632

Pathways: Apoptosis