alpha Adaptin antibody (C-Term)

Catalog No. ABIN2788287

The Rabbit Polyclonal anti-alpha Adaptin antibody (ABIN2788287) specifically detects alpha Adaptin in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Pig, Horse, Rabbit and Guinea Pig samples.

Quick Overview for alpha Adaptin antibody (C-Term) (ABIN2788287)

Target: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Adaptin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-alpha Adaptin Antibody

Binding Specificity: C-Term

Purpose: AP2A1 Antibody - C-terminal region

Sequence: ILAKLKRKKG PGAGSALDDG RRDPSSNDIN GGMEPTPSTV STPSPSADLL

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against AP2A1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human AP2A1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP59972-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for alpha Adaptin

Target: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Alternative Name: AP2A1

Background:

Targeted Subunit: alpha-1

Background Information: This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.

Gene Name: Adaptor-related protein complex 2, alpha 1 subunit

Alternative Symbols: ADTAA, CLAPA1, AP2-ALPHA

Protein Name: AP-2 complex subunit alpha-1

Molecular Weight: 105kDa

Gene ID: 160

NCBI Accession: NP_570603

UniProt: O95782

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation