NLRP3 antibody (N-Term)

Catalog No. ABIN2789437

This Rabbit Polyclonal antibody specifically detects NLRP3 in WB. It exhibits reactivity toward Human, Rabbit, Cow, Dog, Pig and Horse.

Quick Overview for NLRP3 antibody (N-Term) (ABIN2789437)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reactivity: Human, Rabbit, Cow, Dog, Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NLRP3 Antibody

Binding Specificity: N-Term

Purpose: NLRP3 Antibody - N-terminal region

Sequence: LGESVSLNKR YTRLRLIKEH RSQQEREQEL LAIGKTKTCE SPVSPIKMEL

Predicted Reactivity: Cow: 93%, Dog: 79%, Horse: 93%, Human: 100%, Pig: 93%, Rabbit: 79%

Characteristics: This is a rabbit polyclonal antibody against NLRP3. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP63297-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NLRP3

Background:

Background Information: This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID).

Gene Name: NLR family, pyrin domain containing 3

Alternative Symbols: AII, AVP, FCU, MWS, FCAS, KEFH, CIAS1, FCAS1, NALP3, C1orf7, CLR1.1, DFNA34, PYPAF1, AGTAVPRL

Protein Name: NACHT, LRR and PYD domains-containing protein 3

Molecular Weight: 114kDa

Gene ID: 114548

NCBI Accession: NP_004886

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome