HLA-DMB antibody (N-Term)

Catalog No. ABIN2789474

This Rabbit Polyclonal antibody specifically detects HLA-DMB in WB and IHC. It exhibits reactivity toward Human and Rat.

Quick Overview for HLA-DMB antibody (N-Term) (ABIN2789474)

Target: HLA-DMB (Major Histocompatibility Complex, Class II, DM beta (HLA-DMB))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DMB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HLA-DMB Antibody

Binding Specificity: N-Term

Sequence: FTYCISFNKD LLTCWDPEEN KMAPCEFGVL NSLANVLSQH LNQKDTLMQR

Predicted Reactivity: Human: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against HLA-DMB. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 263 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HLA-DMB

Target: HLA-DMB (Major Histocompatibility Complex, Class II, DM beta (HLA-DMB))

Alternative Name: HLA-DMB

Background: HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
Alias Symbols: D6S221E, RING7
Protein Interaction Partner: CUL3, TEAD1, MDM2, DPM2, CD82, HLA-DOB, CD63, HLA-DRA, HLA-DMA, CD74,
Protein Size: 263

Molecular Weight: 29 kDa

Gene ID: 3109

NCBI Accession: NM_002118, NP_002109

UniProt: P28068

Pathways: Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response