JMY antibody (N-Term)

Catalog No. ABIN2789961

Product Details anti-JMY Antibody

Target: JMY (Junction Mediating and Regulatory Protein, P53 Cofactor (JMY))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JMY antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VFIVAWNEIE GKFAITCHNR TAQRQRSGSR EQAGARGGAE AGGAASDGSR

Predicted Reactivity: Cow: 86%, Guinea Pig: 93%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against JMY. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 712 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for JMY

Target: JMY (Junction Mediating and Regulatory Protein, P53 Cofactor (JMY))

Alternative Name: JMY (JMY Products)

Synonyms: WHDC1L3 antibody, junction mediating and regulatory protein, p53 cofactor antibody, junction-mediating and regulatory protein antibody, JMY antibody, jmy antibody, Jmy antibody

Background: JMY acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, JMY acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. JMY increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, JMY acts as a nucleation-promoting factor for both branched and unbranched actin filaments. JMY activates the Arp2/3 complex to induce branched actin filament networks. JMY also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. JMY contributes to cell motility by controlling actin dynamics. JMY may promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.
Alias Symbols: FLJ37870, MGC163496, WHDC1L3
Protein Interaction Partner: CCDC136, UBC, EP300, MDM2, E2F4, E2F3, E2F2, E2F1,
Protein Size: 712

Molecular Weight: 82 kDa

Gene ID: 133746

NCBI Accession: NM_152405, NP_689618

UniProt: Q8N9B5

Pathways: Regulation of Actin Filament Polymerization