DCLK1 antibody (C-Term)

Catalog No. ABIN2789984

The Rabbit Polyclonal anti-DCLK1 antibody (ABIN2789984) specifically detects DCLK1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit and Dog samples.

Quick Overview for DCLK1 antibody (C-Term) (ABIN2789984)

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCLK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DCLK1 Antibody

Binding Specificity: C-Term

Purpose: DCLK1 Antibody - C-terminal region

Sequence: IFIACGPEKF RYQDDFLLDE SECRVVKSTS YTKIASSSRR STTKSPGPSR

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against DCLK1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human DCLK1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP64869-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DCLK1

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Alternative Name: DCLK1

Background:

Background Information: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.

Gene Name: doublecortin like kinase 1

Alternative Symbols: CL1, DCLK, CLICK1, DCDC3A, DCAMKL1

Protein Name: serine/threonine-protein kinase DCLK1

Molecular Weight: 39kDa

Gene ID: 9201

NCBI Accession: NP_001182344

UniProt: O15075