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Anti-MBP-1 (Middle Region) antibody

Reactivity: Human IHC, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2792670
  • Target
    Anti-MBP-1
    Binding Specificity
    Middle Region
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Immunohistochemistry (IHC), Western Blotting (WB)
    Sequence
    GSGGMTHSDQ PKEDRQGVNE KSCNCLLLKV NQIGSVTESL QACKLAQANG
    Cross-Reactivity
    Human
    Characteristics
    This is a rabbit polyclonal antibody against ENO1. It was validated on Western Blot using a cell lysate as a positive control.
    Immunogen
    The immunogen is a synthetic peptide directed towards the middle region of human ENO1
  • Application Notes
    Antibody Dilution: 0.5 µg/mL.
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 434 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    Anti-MBP-1
    Target Type
    Antibody
    Background
    ENO1 encodes one of three enolase isoenzymes found in mammals, it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
    Alias Symbols: NNE, PPH, MPB1, ENO1L1
    Protein Interaction Partner: UBC, FUS, HUWE1, ISG15, SUMO2, SUMO3, IVNS1ABP, STAU1, MDM2, WWOX, EED, FBXO6, UPF2, YWHAQ, UBD, TERT, SRC, RAD52, NPM1, BHLHE40, VCAM1, HCVgp1, ITGA4, FN1, ATF2, SERPING1, AMBP, LIG4, BRCA1, MDC1, PGK1, ITPA, GAPDH, FYN, FLNC, ENO3, Htt, TPI1, PPIA, AKT1
    Protein Size: 434
    Molecular Weight
    47 kDa
    Gene ID
    2023
    NCBI Accession
    NM_001428, NP_001419
    UniProt
    P06733
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