PUMA antibody (BH3 Domain)

Catalog No. ABIN2798057

The Rabbit Polyclonal anti-PUMA antibody has been validated for WB, IHC, IF and FACS. It is suitable to detect PUMA in samples from Human.

Quick Overview for PUMA antibody (BH3 Domain) (ABIN2798057)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-PUMA Antibody

Binding Specificity: AA 119-154, BH3 Domain

Purpose: Rabbit Anti-Human Puma BH3 Domain Antibody

Immunogen: This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50, IF: 1:10-50, FC: 1:10-50Western blot analysis of anti-Puma BH3 domain Pab .FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.000 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: Puma

Background:

Target Description: PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Gene Symbol: BBC3

Molecular Weight: 20532 Da

Gene ID: 27113

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity