MAPK11 antibody (Mitogen-Activated Protein Kinase 11) (AA 1-30)

Details for Product anti-MAPK11 Antibody No. ABIN2798348, Supplier: Log in to see
Antigen
  • ATMPK11
  • F22L4.10
  • F22L4_10
  • MAP kinase 11
  • zgc:86905
  • P38B
  • P38BETA2
  • PRKM11
  • SAPK2
  • SAPK2B
  • p38-2
  • p38Beta
  • P38b
  • Prkm11
  • Sapk2
  • Sapk2b
  • p38beta
  • p38beta2
  • p38b
  • sapk2
  • prkm11
  • sapk2b
  • mitogen-activated protein kinase 11
  • MAP kinase 11
  • mapk11
  • LOC9328118
  • MPK11
  • MAPK11
  • Mapk11
  • PTRG_02565
Alternatives
anti-Human MAPK11 antibody for Western Blotting
Epitope
AA 1-30, N-Term
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13
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11
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8
5
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2
1
1
1
Reactivity
Mouse (Murine)
157
88
51
2
2
1
Host
Rabbit
137
23
3
Clonality
Polyclonal
Conjugate
This MAPK11 antibody is un-conjugated
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2
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2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
124
70
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26
14
9
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4
3
2
2
2
1
1
Options
Supplier
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Supplier Product No.
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'Independent Validation' Badge
Antigen MAPK11
Lot Number RAY8110713
Method validated Western Blotting
Positive Control primary mouse microglia
Negative Control siRNA knock-down of MAPK11 in primary mouse microglia
Notes Passed. ABIN2798348 specifically recognizes Mapk11 in primary mouse microglia lysates.
Primary Antibody ABIN2798348
Secondary Antibody anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
Protocol
  • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
  • Transfect cells with 0.05nmol mouse MAPK11 Silencer Select siRNA (ThermoFisher Scientific, Sample ID ASO10943, ASO1098J and ASO109CX, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer´s instructions.
  • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma).
  • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
  • Denature 5µg total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing freshly cast 10% SDS-PAGE for 1h at 140V.
  • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
  • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
  • Incubation with primary rabbit anti-MAPK11 antibody (antibodies-online, ABIN2798348, lot RAY8110713) diluted 1:500 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST.
  • Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
  • Reveal protein bands using ECL Prime Western Blotting Detection Reagent (GE Healthcare, RPN2232, lot 9547481) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
  • Strip membranes RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
  • Wash and reveal the protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
Experimental Notes The MAPK11 antibody ABIN2798348 reveals a protein of the expected molecular weight of murine Mapk11 in lysates of primary mouse microglia. The protein band’s intensity decreases upon siRNA knockdown.
Validation Images
Western Blotting validation image for anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (N-Term), (AA 1-30) antibody (ABIN2798348) Primary mouse microglia were transfected with Mapk11 siRNA and Mapk11 was revealed us...
Purpose Rabbit Anti-Mouse Mapk11 (N-term) Antibody
Immunogen This Mouse Mapk11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of mouse Mapk11.
Isotype Ig Fraction
Plasmids, Primers & others Plasmids, Primers & others MAPK11 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Mapk11 (MAPK11 Antibody Abstract)
Background

Target Description: Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2 (By similarity).

Gene Symbol: Mapk11

Molecular Weight 41397 Da
Gene ID 19094
UniProt Q9WUI1
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Response to Water Deprivation, Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Hepatitis C, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling
Application Notes Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50Western blot analysis of lysate from mouse NIH/3T3 cell line, using Mouse Mapk11 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Storage 4 °C,-20 °C
Storage Comment 2-8°C (short-term), -20°C (long-term)
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