PIDD antibody (AA 551-650) (AbBy Fluor® 594)

Catalog No. ABIN2803836

The Rabbit Polyclonal anti-PIDD antibody has been validated for IF (cc) and IF (p). It is suitable to detect PIDD in samples from Rat.

Quick Overview for PIDD antibody (AA 551-650) (AbBy Fluor® 594) (ABIN2803836)

Target: PIDD (P53-Induced Death Domain Protein (PIDD))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIDD antibody is conjugated to AbBy Fluor® 594

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-PIDD Antibody (AbBy Fluor® 594)

Binding Specificity: AA 551-650

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LRDD/PIDD

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PIDD

Target: PIDD (P53-Induced Death Domain Protein (PIDD))

Alternative Name: LRDD

Background:

Synonyms: Leucine rich repeats and death domain containing, Leucine-rich repeats and death domain containing, MGC16925, p53-induced protein with a death domain, PIDD, DKFZp434D229, PIDD_HUMAN.

Background: The death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression. Independently, PIDD was also described as a DD-containing protein with unknown function. The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, followed by two ZU-5 domains and a C-terminal DD. PIDD forms a complex with caspase-2 and the adaptor protein RAIDD. Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli, via interaction with caspase-2 and CRADD/RAIDD. PIDD also promotes apoptosis downstream of p53 as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. PIDD has also been shown to interact with NEMO/IKBKG and RIP1 and enhance sumoylation and ubiquitination of NEMO/IKBKG, an important component for activation of the transcription factor NF-kappa-B.

Pathways: p53 Signaling, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity