PIDD antibody (AA 776-910)

Catalog No. ABIN1169268

Cited in 3+ publications. The Rabbit Polyclonal anti-PIDD antibody (ABIN1169268) specifically detects PIDD in IHC, WB and IP. The antibody is reactive with Human and Mouse samples.

Quick Overview for PIDD antibody (AA 776-910) (ABIN1169268)

Target: PIDD (P53-Induced Death Domain Protein (PIDD))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIDD antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-PIDD Antibody

Binding Specificity: AA 776-910

Purpose: anti-PIDD, pAb (AL233)

Specificity: Recognizes human and the native form of mouse PIDD. Detects bands of ~90 kDa (Full Length FL-PIDD) and ~50 kDa (cleaved protein) by Western blot.

Characteristics: Polyclonal Antibody. Recognizes human and the native form of mouse PIDD. Detects bands of ~90 kDa (full length protein) and ~50 kDa (cleaved protein) by Western blot. Source: Rabbit. Applications: IHC, IP, WB. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. PIDD promotes apoptosis downstream of the tumor suppressor as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. It associates with NEMO/IKBKG and RIP1 and enhances sumoylation and ubiquitination of NEMO/IKBKG which is important for activation of the transcription factor NF-kappa-B. It associates with caspase-2 and RAIDD, and induces activation of caspase-2 which is an important regulator in the apoptotic pathways.

Immunogen: Recombinant human PIDD (Death Domain: aa 776-910).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-PIDD antibody (ABIN1169268)

Milleron, Bratton: "Heat shock induces apoptosis independently of any known initiator caspase-activating complex." in: The Journal of biological chemistry, Vol. 281, Issue 25, pp. 16991-7000, (2006) (PubMed).

Janssens, Tinel, Lippens, Tschopp: "PIDD mediates NF-kappaB activation in response to DNA damage." in: Cell, Vol. 123, Issue 6, pp. 1079-92, (2005) (PubMed).

Tinel, Tschopp: "The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress." in: Science (New York, N.Y.), Vol. 304, Issue 5672, pp. 843-6, (2004) (PubMed).

Target Details for PIDD

Target: PIDD (P53-Induced Death Domain Protein (PIDD))

Alternative Name: PIDD

Background:

Alternate Names/Synonyms: p53-induced Protein with a Death Domain, Leucine-rich Repeat and Death Domain-containing Protein, Lrdd

Product Description: PIDD promotes apoptosis downstream of the tumor suppressor as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. It associates with NEMO/IKBKG and RIP1 and enhances sumoylation and ubiquitination of NEMO/IKBKG which is important for activation of the transcription factor NF-kappa-B. It associates with caspase-2 and RAIDD, and induces activation of caspase-2 which is an important regulator in the apoptotic pathways.

UniProt: Q9HB75

Pathways: p53 Signaling, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity