Escherichia Coli O157 antibody

Catalog No. ABIN283323

Product Details

Target: Escherichia Coli O157

Reactivity: E. coli

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF)

Cross-Reactivity (Details): This antibody recognizes the 'O' antigen of E coli serotype O157 Non-reactive with E coli serotypes: O111, O125, O20, O55 & K14

Purification: Affinity chromatography purified

Purity: > 90 % pure

Immunogen: E. coli O157 antibody was raised in mouse using 'O' antigen of E. coli serotype O157 as the immunogen.

Clone: 1516501

Isotype: IgG3

Application Details

Application Notes: ELISA: 1:20-1:200, IF: 1:10-1:50
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: PBS , pH 7.2, with 0.1 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

References

Fu, Rogelj, Kieft: "Rapid detection of Escherichia coli O157:H7 by immunomagnetic separation and real-time PCR." in: International journal of food microbiology, Vol. 99, Issue 1, pp. 47-57, (2005) (PubMed).

Target Details

Target: Escherichia Coli O157

Alternative Name: E. Coli O157

Target Type: Bacteria