Aurora-B (ARK/STK12) (AA 6-35), (N-Term) antibody

Catalog No. ABIN2841374

Product Details

Target: Aurora-B (ARK/STK12)

Binding Specificity: AA 6-35, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Aurora-B (ARK/STK12) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-35 amino acids from the N-terminal region of human Aurora-B (ARK/STK12).

Clone: RB3534

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. IHC-P: 1:50~100. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References

Crowell, Gaffuri, Gayraud-Morel, Tajbakhsh, Echard: "Engulfment of the midbody remnant after cytokinesis in mammalian cells." in: Journal of cell science, Vol. 127, Issue Pt 17, pp. 3840-51, (2014) (PubMed).

Target Details

Target: Aurora-B (ARK/STK12)

Background: Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. STK12 (Aurora kinase B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes

Molecular Weight: 39311

Gene ID: 9212

NCBI Accession: NP_001243763, NP_004208

UniProt: Q96GD4