GFP antibody

Catalog No. ABIN284433

The Mouse Monoclonal anti-GFP antibody (Clone 215) (ABIN284433) specifically detects GFP in WB, ELISA and IHC. The antibody is reactive with Aequorea victoria samples.

Quick Overview for GFP antibody (ABIN284433)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 215

Product Details anti-GFP Antibody

Specificity: The monoclonal detects wild type and recombinant GFP. This antibody does not detect enhanced GFP.

Immunogen: GFP antibody was raised in mouse using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.

Isotype: IgG2a

Application Details

Application Notes: ELISA: 1:200,000, IHC: 1:1,000-1:5,000, WB: 1:3,000-1:30,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot and freeze at -20 °C or below for long term storage. This product is stable for several weeks at 4 °C as an undiluted liquid.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP