Proteasome 26S S3 (Center) antibody
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- Target
- Proteasome 26S S3
- Binding Specificity
- Center
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Cross-Reactivity
- Rhesus Monkey, Chimpanzee, Cow (Bovine)
- Cross-Reactivity (Details)
- Rhesus Monkey (100 %), Chimpanzee (100 %), Bovine (100 %)
- Characteristics
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Rabbit Polyclonal antibody to Proteasome 26S S3 (proteasome (prosome, macropain) 26S subunit, non-ATPase, 3)
Proteasome 26S S3 antibody [C1C3] - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human Proteasome 26S S3. The exact sequence is proprietary.
- Isotype
- IgG
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- Application Notes
- Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*
- Comment
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Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.97 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- Proteasome 26S S3
- Background
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The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid.
- Molecular Weight
- 61 kDa
- Gene ID
- 5709
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