RASSF1 antibody (Center)

Catalog No. ABIN2854789

This Rabbit Polyclonal antibody specifically detects RASSF1 in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for RASSF1 antibody (Center) (ABIN2854789)

Target: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RASSF1 Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Mouse (91 %), Rat (91 %)

Characteristics: Rabbit polyclonal antibody to RASSF1 (Ras association (RalGDS/AF-6) domain family member 1)
RASSF1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RASSF1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , HeLaS3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RASSF1

Target: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Alternative Name: RASSF1

Background: This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Cellular Localization: Isoform A: Cytoplasm , cytoskeleton , Centrosome , Spindle , Isoform C: Nucleus

Molecular Weight: 39 kDa

Gene ID: 11186